Rubisco separation using biocompatible aqueous two-phase systems
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چکیده
منابع مشابه
Rapid One-Step Separation and Purification of Recombinant Phenylalanine Dehydrogenase in Aqueous Two-Phase Systems
Background: Phenylalanine dehydrogenase (PheDH EC 1.4.1.20) is a NAD+-dependent enzyme that performs the reversible oxidative deamination of L-phenylalanine to phenylpyruvate. It plays an important role in detection and screening of phenylketonuria (PKU) diseases and production of chiral intermediates as well. The main goal of this study was to find a simple and rapid alternative method for pur...
متن کاملAqueous two-phase systems for protein separation: phase separation and applications.
Aqueous two-phase systems (ATPS) that are formed by mixing a polymer (usually polyethylene glycol, PEG) and a salt (e.g. phosphate, sulphate or citrate) or two polymers, and water can be effectively used for the separation and purification of proteins. The partitioning between both phases is dependent on the surface properties of the proteins and on the composition of the two phase system as ha...
متن کاملAqueous Two-Phase Systems
ger G and Albertsson PA (1983) AfRnity partitioning of phosphofructokinase from baker’s yeast using polymer-bound Cibacron blue F3GA. European Journal of Biochemistry 131: 589}594. KopperschlaK ger G and Birkenmeier G (1990) AfRnity partitioning and extraction of proteins. Bioseparation 1: 235}254. Tjerneld F, Johansson G and Joelsson M (1987) AfRnity liquid}liquid extraction of lactate dehydro...
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The partitioning of endo-polygalacturonase (endo-PG) in polyethylene glycol (PEG)-polyvinyl alcohol (PVA10000) and PEG-hydroxypropyl starch (Reppal PES100) aqueous two-phase systems was studied, and revealed the possibility of using aqueous two-phase extraction to purify and concentrate endo-PG from its clarified fermentation broth. For the PEG8000-PVA10000 system, endo-PG presented in the ferm...
متن کاملSeparation of methacrylic acid from aqueous phase using quaternary amine
The feasibility of extractive reaction of methacrylic acid from aqueous solution using a quaternary amine, tri–octyl methyl ammonium chloride (TOMAC) as an extractant, was studied. The diluents chosen in the present work belong to different chemical classes, n–butyl acetate, carbon tetrachloride, isoamyl alcohol, methyl isobutyl ketone, and toluene. The effect of initial acid concentration in t...
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ژورنال
عنوان ژورنال: Separation and Purification Technology
سال: 2018
ISSN: 1383-5866
DOI: 10.1016/j.seppur.2017.05.001